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Plasmid#228397PurposeRecombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coliDepositorInsertHopF2 (hopF2 Pseudomonas syringae pv. tomato)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268
Plasmid#227089PurposeBacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)DepositorInserttom1p-HECT (TOM1 Budding Yeast)
UseTagsHIS-MBP-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMYT009
Plasmid#180662PurposeEncodes pLacSpTDH3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacSpTDH3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEGFR-PATagRFP
Plasmid#31950DepositorInsertPATagRFP
UseTagsEGF receptor (EGFR)ExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTfR-PATagRFP
Plasmid#31946DepositorInsertPATagRFP
UseTagsTfRExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVSVG-PATagRFP
Plasmid#31947DepositorInsertPATagRFP
UseTagsVSVGExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-PATagRFP
Plasmid#31945DepositorInsertPATagRFP
UseTagsHisExpressionBacterialMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF3435
Plasmid#144911PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSUPT5H (SUPT5H Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1547
Plasmid#143131PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSUPT5H (SUPT5H Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3467
Plasmid#144943PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSUPT4H1 (SUPT4H1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CPH3486 (YCp LEU2 Rpb1 F1492A)
Plasmid#91808PurposeYeast expression of S. cerevisiae Rpb1 with a F1492A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged phenylalanine 1492 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3487 (YCp LEU2 Rpb1 S1493A)
Plasmid#91809PurposeYeast expression of S. cerevisiae Rpb1 with a S1493A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged serine 1493 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3488 (YCp LEU2 Rpb1 P1494A)
Plasmid#91810PurposeYeast expression of S. cerevisiae Rpb1 with a P1494A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged proline 1494 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3508 (YCp LEU2 Rpb1 Y1473A)
Plasmid#91811PurposeYeast expression of S. cerevisiae Rpb1 with a Y1473A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged tyrosine 1473 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3509 (YCp LEU2 Rpb1 T1471A)
Plasmid#91812PurposeYeast expression of S. cerevisiae Rpb1 with a T1471A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged threonine 1471 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3511 (YCp LEU2 Rpb1 P1472A)
Plasmid#91813PurposeYeast expression of S. cerevisiae Rpb1 with a P1472A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationchanged proline 1472 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
Plasmid#91806PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationResidues A1529-G1534 replaced with a PreScission …PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
Plasmid#91807PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationPreScission Protease site (LEVLFQGP) inserted bet…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
NME8 gRNA (BRDN0001145495)
Plasmid#77115Purpose3rd generation lentiviral gRNA plasmid targeting human NME8DepositorInsertNME8 (NME8 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only