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Plasmid#160212PurposeKnock Down MYDSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat, Human)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA2
Plasmid#160211PurposeKnock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGACGTTGCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA1
Plasmid#160209PurposeKnock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA1
Plasmid#160208PurposeKnock Down MQWSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat, Human)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV2/2
Plasmid#91408PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV2/2DepositorInsertSH3RF2-1/3-SV2/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV1/2
Plasmid#91322PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV1/2DepositorInsertSH3RF2-1/3-SV1/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-hSH3BP4
Plasmid#46895Purposeexpression of shRNA against human SH3BP4DepositorInsertSH3BP4 (SH3BP4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-mSH3BP4
Plasmid#46894Purposeexpression of shRNA against mouse SH3BP4DepositorInsertSH3BP4 (Sh3bp4 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
WT-long-UBASH3A-V5-6xHis
Plasmid#192101PurposeMammalian expression of human wild-type long-form UBASH3A with V5-6xHis tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
UseTags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-CB2 SH3+
Plasmid#68821PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sh3bp2-SH2
Plasmid#46484DepositorInsertSh3bp2 (SH3BP2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-561PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-CTD
Plasmid#136649PurposeExpression of SH3BP1-CTD-GST fusion protein in E. coliDepositorInsertSH3BP1-CTD (SH3BP1 Human)
UseTagsExpressionBacterialMutationContains only C-terminal domainPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-NTD
Plasmid#136648PurposeExpression of SH3BP1-NTD-GST fusion protein in E. coliDepositorInsertSH3BP1-NTD (SH3BP1 Human)
UseTagsExpressionBacterialMutationContains only N-terminal domainPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Endophilin SH3 domains
Plasmid#47401DepositorInsertEndophilin
UseTagsGSTExpressionBacterialMutationSH3 domain (aa 292-352)PromotertacAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _SH3bm
Plasmid#59141PurposeYAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationSH3 domain deleted (aa278-290)PromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn deltaSH3
Plasmid#16034DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDelta SH3: deletion of amino acids 76-141PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-WT-short-UBASH3A
Plasmid#192090PurposeMammalian expression of human wild-type short-form UBASH3A with FLAG tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-CB2 SH3-
Plasmid#68822PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only