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Plasmid#35196DepositorInsertGLT1 POL1 24xPP7SL
UseTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7
Plasmid#196846Purposebackbone for gRNA cloning of dpspCas13b tagged by 2xPP7DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-UP-24xGCN4-2xIFP2-CAAX-24xPP7
Plasmid#134833PurposeExpresses 24xSunTag-2xIFP2-CAAX (24xGCN4_v4-2xIFP2-CAAX)DepositorInsert24xGCN4_v4-2xIFP2-CAAX
UseLentiviralTagsExpressionMutationPromoterdSV40Available sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ416 (24PP7SL loxP-Kan-loxP)
Plasmid#45163DepositorInsert24xPP7 Stem Loop Cassette
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
UseTagskanMXExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-RPS27A-UTR-PP7-WPRE
Plasmid#198339PurposePB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loopsDepositorInsertRPS27A-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loopsExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-boxB-MS2-PP7
Plasmid#75397PurposesgRNA1-boxB-MS2-PP7DepositorInsertsgRNA1-boxB-MS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
Plasmid#45931DepositorInsertPCP-PS
UseTags2x-yeGFPExpressionYeastMutationPromoterMet25Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-SunTag-RPS7-PP7-WPRE
Plasmid#198338PurposePB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loopsDepositorInsertGCN4x24-RPS7-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loops and SunTagExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7 oligo-library based
Plasmid#158199PurposeCassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of PP7 from the oligo library
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7
Plasmid#74931PurposeTranslation reporter with Xbp1 pause siteDepositorInsert24xGCN4_v4-kif18b-Xbp1-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)
Plasmid#189792PurposeExpress wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ514 pURA MET25p PP7-PS-2x-yeGFP
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ536 pURA ADE3p PP7-PS-2x-yeGFP
Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP
Plasmid#72233PurposeTryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only