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pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Use

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Use

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pBiFC-HA-hCaspase 2 FL (C303S)-VC155

Plasmid

#49257

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
rtTA-sg-mGreenLantern/EGFP

Plasmid

#188482

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mNeonGreen-EC18153

Plasmid

#137075

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Use

Tags

ymNeonGreen

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mCherry

Plasmid

#125700

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mTurquoise2

Plasmid

#125701

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mTurquoise

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mNeonGreen-HMG-15b

Plasmid

#206872

Purpose

Express mNeonGreen-HMG

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

mNeonGreen-HMG

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mNeonGreen-15b

Plasmid

#206870

Purpose

Express tTALE-mNeonGreen

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern/EGFP

Plasmid

#179913

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-URA3

Plasmid

#168059

Purpose

Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Use

Tags

ymNeonGreen

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Use

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mNeonGreen-WPRE

Plasmid

#193334

Purpose

Expresses mNeonGreen under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen-WPRE

Plasmid

#193341

Purpose

AAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mGreenLantern

Plasmid

#164469

Purpose

Constitutive CAG-driven expression of mGreenLantern in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

AAV

Tags

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CAG

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-DIO-mGreenLantern

Plasmid

#164468

Purpose

Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

AAV

Tags

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

EF1a

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen

Plasmid

#124101

Purpose

Expression of the fluorescent protein mNeonGreen in mammalian cells

Depositor

Pantelis Tsoulfas

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Codon usage was optimized for Homo Sapiens

Promoter

CAG

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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