Showing: 81 - 100 of 127 results
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Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_LMNA_G2
Plasmid#178090PurposeExpresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.DepositorInsertLMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorInsertSMC4-mAID-HaloTag (SMC4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ntng2.a-AP-His
Plasmid#71994PurposeExpresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtng2.a (Ntng2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-AP-His
Plasmid#71995PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Ntng2.a-Fc-His
Plasmid#72120PurposeExpresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng2.a (Ntng2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-Fc-His
Plasmid#72121PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2
Plasmid#86610PurposeExpresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmidDepositorInsertATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
Hygro-P2A-HA-FKBP-WAPL
Plasmid#217666PurposeRepair template to endogenously tag WAPL with Hygro-P2A-HA-FKBPDepositorInsertWapI (WAPL Human)
UseTagsdTag (FKBP36V protein)ExpressionBacterial and MammalianMutationPromoterLacAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA
Plasmid#86612PurposeVector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
Plasmid#83842PurposeFluorescent probe for S/G2 cell cycle transition and G2/M transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7AvailabilityAcademic Institutions and Nonprofits only -
pCS2+CyclinB1-GFP
Plasmid#128426PurposeContains insert allowing in-vivo visualization of CyclinB1 protein (G2/M)DepositorInsertCyclinB1-GFP
UseTagsExpressionMammalianMutationPromoterCMV & SP6AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Vx3(A7)
Plasmid#35526DepositorInsertVx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pOC2 CRISPIE-Halo-Homer1 KI
Plasmid#183446PurposeCreON knock-in for Halo-Homer1 (amino acid position: G2)DepositorInsertgRNA and CRISPIE Halo donor
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 127 results