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NPC1 His6 ECFP

Plasmid

#53522

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Use

Tags

ECFP and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
RPL13-ECFP-SNAP

Plasmid

#187967

Purpose

expresses RPL13 with C-terminal tags ECFP and SNAP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

RPL13 (RPL13 Human)

Use

Tags

ECFP-SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-ECFP

Plasmid

#48353

Purpose

Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

ECFP

Use

Gateway entry vector

Tags

Expression

Mutation

Contains a stop codon.

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Mtp-p53-ECFP

Plasmid

#53961

Purpose

Expresses CFP tagged p53 in mammalian cells

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

p53 (TP53 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Use

Tags

ECFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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Vimentin - APEX2 in pECFP

Plasmid

#66170

Purpose

APEX2 fused to the C-terminus human vimentin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

human Vimentin - APEX2 (VIM Synthetic, Human)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-SECFP-Galectin3

Plasmid

#64150

Purpose

Expression in mammalian cells (production of retrovirus vector)

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Retroviral

Tags

SECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pFX-H2B-SECFP

Plasmid

#175838

Purpose

Express SECFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pECFP-IETD2x-Venus

Plasmid

#24536

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-IETD2x-Venus

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
lvl0 eCFP CDS

Plasmid

#153397

Purpose

Cyan fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eCFP CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Clathrin-LCa-ECFP

Plasmid

#21742

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
LCMV:ECFP(loxP)EYFP

Plasmid

#31306

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)EYFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Epsin1 deltaUIM-ECFP

Plasmid

#20973

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin1 deltaUIM (Epn1 Rat)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2009

Availability

Academic Institutions and Nonprofits only
Flag-ECFP-GIT1

Plasmid

#15223

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Use

Tags

ECFP and flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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