-
Plasmid#130983PurposeBacterial expression of mouse EB3-GFP-6xHis for protein purification.DepositorInsertEB3-GFP-6xHis (Mapre3 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB31 (hANGPTL8-V5-His lenti)
Plasmid#109116PurposeV5 and His-tagged human ANGPTL8 in a lentiviral vectorDepositorInsertANGPTL8 (ANGPTL8 Human)
UseLentiviralTags6x His and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorInsertEB3-mCherry-6xHis (Mapre3 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-EB3-NeonGreen
Plasmid#175263Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3 (MAPRE3 Human)
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EB3-eGFP-2xC2-iLID
Plasmid#114419Purposeanchor iLID domain to plasma membrane with slower diffusionDepositorInsertiLID domain
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-control -- AEB3mRFP1
Plasmid#63851PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAEB3Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-(FspAI)-mCherry-PH (EB3)
Plasmid#206472PurposeExpression in Schneider cells for imagingDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-034 EB3(D-G)//Esp3I
Plasmid#130236PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-EB3N-VVDfast-mCl3-iLID
Plasmid#190169PurposeDoxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseLentiviralTags-VVDfast-mClover3-iLIDExpressionMutation1-189 aa from EB3PromoterTREAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-028 EB3-mVenus//BsaI
Plasmid#130209PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-031 EB3-Eos//BsaI
Plasmid#130212PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-014 EB3-mVenus//BsaI
Plasmid#130195PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-013 EB3-Eos//BsaI
Plasmid#130194PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-007 EB3-SuperNova//BsaI
Plasmid#130188PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-SuperNova
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-006 EB3-Sirius//BsaI
Plasmid#130187PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]
Plasmid#196885PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry
Plasmid#175261Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
UseTagsmCherry (on iLifeact) and mNeonGreen (on EB3)ExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA
Plasmid#113697PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]
Plasmid#196887PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDK712
Plasmid#134207PurposeMammalian expression of EB3-tDT BMAMDepositorInsertEB3-tDT BMAM
UseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only