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Plasmid#210938PurposeInsect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertEIF2A:M1-T427 (EIF2A Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_COPB2:1-300
Plasmid#210917PurposeInsect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCOPB2:M1-G300 (COPB2 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_PRPF4:1-522
Plasmid#210896PurposeInsect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR12:90-422
Plasmid#210893PurposeInsect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR12:T90-G422 (WDR12 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR41:30-459
Plasmid#210892PurposeInsect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR41:Q30-V459 (WDR41 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_CORO1A:1-461
Plasmid#210891PurposeInsect expression of full-length human CORO1A. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCORO1A:M1-K461 (CORO1A Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_RACK1:1-317
Plasmid#210889PurposeInsect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR91:1-747
Plasmid#210879PurposeInsect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR91:M1-A747 (WDR91 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
1533_pAAV-CB-AcGFP-BirA-HA
Plasmid#208201PurposeControl for FKBP12-BirA constructDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterChicken beta actinAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
1529_pAAV-CB-FKBP12-BirA-HA
Plasmid#208200PurposeExpress the murine FKBP12-BirA-HA fusion protein in AAV vectorDepositorInsertFkbp12 (Fkbp1a Mouse)
UseAAVTagsExpressionMutationPromoterChicken beta-actinAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* zeo
Plasmid#192332PurposeLentiviral expression vector for an inducible 3xFLAG-BirADepositorInsertBirA*
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT BirA* w ccdB
Plasmid#192310PurposeGateway entry vector for cloningDepositorInsertBirA*
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-birA*
Plasmid#179788PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertbirA
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago2
Plasmid#170918PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago1
Plasmid#170919PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA-BirA*-pDEST-N-pcDNA5/FRT/TO
Plasmid#118375PurposeExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.DepositorTypeEmpty backboneUseDestinationTagsHA-BirA*ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6-MBP-TEV-BirA
Plasmid#179694PurposeExpresses His6 tagged MBP-BirA fusion proteinDepositorInsertBirA
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon optiā¦PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only