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Showing: 81 - 100 of 2545 results

  2. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

    Plasmid
    #178100
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178100-plasmid-map-sequence-id-350130

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  4. cfSBFP2-N

    Plasmid
    #110633
    Purpose
    Cys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.
    Depositor
    Ikuo Wada
    Insert
    cfSBFP2
    Use
    Tags
    Expression
    Mutation
    Cys48Ser/Cys70Met
    Promoter
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    110633-plasmid-map-sequence-id-291909

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  8. LCV2_BFP2

    Plasmid
    #155097
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155097-plasmid-map-sequence-id-308002

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  13. psecBFP2

    Plasmid
    #62230
    Purpose
    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
    Depositor
    Erik Snapp
    Article
    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;
    Insert
    secBFP2
    Use
    Tags
    secbfp2
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

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  19. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

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  20. pDual_dsCas9_BFP

    Plasmid
    #214679
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214679-plasmid-map-sequence-id-428808

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Showing: 81 - 100 of 2545 results