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Plasmid#219643PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest methodDepositorInsertmGreenLantern SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64
Plasmid#85423PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-5 synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
UseTagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64
Plasmid#85425PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-8_synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiEEscFv-TM
Plasmid#198304PurposeDisplays anti-EE scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiEEscFv-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64
Plasmid#85422PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic NotchDepositorInsertanti-Her_4D5-3 synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PDZD8HA_sh_resistant
Plasmid#105006PurposeMammalian Expression of PDZD8, sh resistantDepositorInsertPDZD8 (Pdzd8 Mouse)
UseTagsHAExpressionMammalianMutationThe sequence recognized by shPDZD8 (TRCN000043105…PromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST exorh:mGreenLantern
Plasmid#195988PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-FX(a) Emi HC
Plasmid#113665PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FX/FXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP1-WW mutant
Plasmid#17792DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 to Alanine; Proline 202 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNTD_P2A_Hygro_Barcode
Plasmid#120510PurposeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaNTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of amino-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER2-mGreenLantern
Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiMoonTagNb-TM
Plasmid#198305PurposeDisplays anti-MoonTag nanobody from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiMoonTagNb-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 4
Plasmid#188364PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Gli3_Anti-Sense
Plasmid#124440PurposePlasmid for anti-sense in situ probe in vitro transcriptionDepositorInsertGLI-Kruppel family member GLI3 (Gli3 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiSunTag-TM
Plasmid#198306PurposeDisplays anti-SunTag scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiSunTag-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Antp
Plasmid#86866PurposeBacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.DepositorInsertLinker-Antp
UseTagsAntpExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mGreenLantern
Plasmid#164467PurposeVisualization of the ER in cells (compatible with OSER assay)DepositorInsertmGreenLantern
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_Giantin_C1
Plasmid#85049PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only