1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. PX459_GRHL1-exon5

   Plasmid

   #177763

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459_GRHL1-exon8

   Plasmid

   #177764

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459v2-ILK-gRNA 1-exon 1

   Plasmid

   #163320

   Purpose

   SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ILK gRNA 1_Exon1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

4. PX459v2-ILK-gRNA 2-exon 8

   Plasmid

   #163321

   Purpose

   SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ILK gRNA 2_Exon8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6F

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

5. Rab11 single guide RNA

   Plasmid

   #229683

   Purpose

   guide RNA for Rab11 tagging at the N-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   Rab11 guide (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

   Plasmid

   #195504

   Purpose

   Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT9679-sgRNA-BFP

   Plasmid

   #162988

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX459-CTNNA1(α-catenin CRISPR KO)

   Plasmid

   #229708

   Purpose

   Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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