Addgene

We narrowed to 55,745 results for: %s

Showing: 81 - 100 of 55745 results

Results per page:

Sort results by:

phU1-PspCas13b-non-targeting (pEZ36)

Plasmid

#229053

Purpose

Cloning backbone for PspCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Cas13bt1-non-targeting (pJK2)

Plasmid

#229054

Purpose

Cloning backbone for Cas13bt1 crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
phU1-Cas13bt1-non-targeting (pEZ25)

Plasmid

#229055

Purpose

Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-RanCas13b-non-targeting (pJK6)

Plasmid

#229056

Purpose

Cloning backbone for RanCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
phU1-RanCas13b-non-targeting (pEZ45)

Plasmid

#229057

Purpose

Cloning backbone for RanCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pmirGLO-mir1-8mer4x (pEZ184)

Plasmid

#229058

Purpose

Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-1

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

Human miR-1 binding sites

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI5-CasRx-HA (pKW88)

Plasmid

#229046

Purpose

Mammalian expression of CasRx-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJD1

Plasmid

#222374

Purpose

Recombinant human IgG1 antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF125

Plasmid

#222373

Purpose

Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG (partial)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF201

Plasmid

#222376

Purpose

Recombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF200

Plasmid

#222375

Purpose

Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Plasmid

#217514

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Use

Tags

smGdP-10XOllas

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Use

Tags

ALFA tag

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mAMCase_CatD_6xHis_pTwistCMV

Plasmid

#200228

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mAMCase_CatD_6xHis_pcDNA3.1

Plasmid

#200229

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSP1D12W/2Y

Plasmid

#124275

Purpose

Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

Depositor

Alexey S. Ladokhin

Insert

Membrane scaffold protein

Use

Tags

7xHis-tag

Expression

Bacterial

Mutation

Two tryptophans changed to two tyrosines

Promoter

T7

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only