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Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
UseTagsExpressionBacterialMutationPromoterpFAB4005Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p006-Banana-Late
Plasmid#112251PurposeATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.DepositorInsertATF1 under osmY promoter
UseTagsExpressionBacterialMutationPromoterosmYAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-4
Plasmid#111478Purposetranslational release factor 1DepositorInserttranslational release factor 1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
B1-4
Plasmid#111466Purposetranslational elongation factor TuDepositorInserttranslational elongation factor Tu
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-1
Plasmid#111475PurposeAla-tRNA synthetaseDepositorInsertAla-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B7-5
Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B1-3
Plasmid#111465Purposetranslational release factor 3DepositorInserttranslational release factor 3
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B1-5
Plasmid#111467Purposetranslational elongation factor TsDepositorInserttranslational elongation factor Ts
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-2
Plasmid#111472Purposetranslational elongation factor 4DepositorInserttranslational elongation factor 4
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B7-1
Plasmid#111489Purposetranslational elongation factor GDepositorInserttranslational elongation factor G
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
B5-5
Plasmid#111484Purposetranslational initiation factor 3DepositorInserttranslational initiation factor 3
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-1
Plasmid#111471Purposetranslational initiation factor 2DepositorInserttranslational initiation factor 2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-5
Plasmid#111479Purposeribosome recycling factorDepositorInsertribosome recycling factor
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1-1
Plasmid#111463PurposeVal-tRNA synthetaseDepositorInsertVal-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
B1-2
Plasmid#111464PurposeMet-tRNA synthetaseDepositorInsertMet-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
B7-4
Plasmid#111492PurposeMet-tRNA formyltransferaseDepositorInsertMet-tRNA formyltransferase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-4
Plasmid#111488PurposePhe-tRNA synthetase ADepositorInsertPhe-tRNA synthetase A
UseTags6xHisExpressionBacterialMutationA81SPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B7-3
Plasmid#111491PurposeTyr-tRNA synthetaseDepositorInsertTyr-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-1
Plasmid#111485PurposePhe-tRNA synthetase BDepositorInsertPhe-tRNA synthetase B
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-2
Plasmid#111486PurposePro-tRNA synthetaseDepositorInsertPro-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-3
Plasmid#111487PurposeSer-tRNA synthetaseDepositorInsertSer-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-2
Plasmid#111481PurposeArg-tRNA synthetaseDepositorInsertArg-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B3-3
Plasmid#111473PurposeGlu-tRNA synthetaseDepositorInsertGlu-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-3
Plasmid#111470PurposeLys-tRNA synthetaseDepositorInsertLys-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-4
Plasmid#111483PurposeTrp-tRNA synthetaseDepositorInsertTrp-tRNA synthetase
UseTags5xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-2
Plasmid#111469PurposeThr-tRNA synthetaseDepositorInsertThr-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-2
Plasmid#111476PurposeAsp-tRNA synthetaseDepositorInsertAsp-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-3
Plasmid#111482PurposeCys-tRNA synthetaseDepositorInsertCys-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-3
Plasmid#111495PurposeHis-tRNA synthetaseDepositorInsertHis-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-1
Plasmid#111494PurposeGly-tRNA synthetase BDepositorInsertGly-tRNA synthetase B
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-4
Plasmid#111496PurposeGly-tRNA synthetase ADepositorInsertGly-tRNA synthetase A
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only