Showing: 201 - 250 of 1866 results
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Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)
Plasmid#185491PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)
Plasmid#185492PurposeCMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10APromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailabilityAcademic Institutions and Nonprofits only -
Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
Plasmid#12474DepositorInsertTop7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
UseTags6xHisExpressionBacterialMutationb19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210735PurposeCoding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Gollum sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210736PurposeCoding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Merry sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailabilityAcademic Institutions and Nonprofits only -
PROM_10
Plasmid#201301PurposeD10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM partDepositorInsertD10: Promoter of TraesCS3D02G517600
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailabilityAcademic Institutions and Nonprofits only -
2P65
Plasmid#60113PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPv089580:MAC01Q-D10:C26843
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMT-TAK1-V5-His
Plasmid#63753PurposeExpresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertTGF-β activated kinase 1 (Tak1 Fly)
UseTagsV5-HisExpressionInsectMutationPromoterpMTAvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR139
Plasmid#106044PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)DepositorInsertOpto-GPR139
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265L2
Plasmid#174000PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N2
Plasmid#174001PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O2
Plasmid#174002PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pH-ABE8e
Plasmid#200774PurposeFor plant adenine base editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(D10A)-TadA8e
UseCRISPRTagsExpressionPlantMutationD10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…Promotermaize Ubiquitin-1, OsU3AvailabilityAcademic Institutions and Nonprofits only -
pH-ABE8e-spG
Plasmid#200775PurposeFor plant adenine base editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9-spG(D10A)-TadA8e
UseCRISPRTagsExpressionPlantMutationD10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…Promotermaize Ubiquitin-1, OsU3AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pORFE0002
Plasmid#112071PurposeAtCAS9 nickase D10A module CDS1DepositorInsertAtCAS9 nickase D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoterAvailabilityAcademic Institutions and Nonprofits only -
pORFE0003
Plasmid#112072PurposeAtDeadCAS9 D10A H840A module CDS1DepositorInsertAtDeadCAS9 D10A H840A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailabilityAcademic Institutions and Nonprofits only -
pORFE0014
Plasmid#112076PurposeAtCAS9 nickase D10A module CDS1nsDepositorInsertAtCAS9 D10A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailabilityAcademic Institutions and Nonprofits only -
pITI2
Plasmid#166095PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailabilityAcademic Institutions and Nonprofits only -
pPH3
Plasmid#166098PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8eWQ
Plasmid#161815PurposeExpresses ABE8eWQ in mammalian cellsDepositorInsertbpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ151
Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationD10A mutation; NLS fusionPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pYPQ265N1
Plasmid#173998PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10APromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O1
Plasmid#173999PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10APromoterAvailabilityAcademic Institutions and Nonprofits only -
pNW3
Plasmid#53372PurposeCsy4 and Cas9 D10A nickase expression plasmidDepositorInsertCsy4-T2A-Cas9n (D10A)
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0201
Plasmid#91002PurposeModule A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0202
Plasmid#91003PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterAtUbi10AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0208
Plasmid#91004PurposeModule A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterEC1.2AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1210
Plasmid#91033PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSPDepositorInsertTaCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterZmUbiAvailabilityAcademic Institutions and Nonprofits only -
AIO-GFP
Plasmid#74119PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265K
Plasmid#173997PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationR33A in rAPOBEC1 and D10A in zCas9PromoterAvailabilityAcademic Institutions and Nonprofits only -
c3GIC9n
Plasmid#62192Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9 (D10A)
UseCRISPRTagsHAExpressionMutationD10A substitution (Cas9n)PromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0408
Plasmid#91010PurposeModule A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterEC1.2AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0601
Plasmid#91016PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0608
Plasmid#91018PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterEC1.2AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0801
Plasmid#91022PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0802
Plasmid#91023PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10AvailabilityAcademic Institutions and Nonprofits only
Showing: 201 - 250 of 1866 results