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Showing: 121 - 150 of 322 results

  1. pET19b-EcMscL-TEV-mEGFP

    Plasmid
    #165097
    Purpose
    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
    Depositor
    Neha Kamat
    Article
    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p
    Insert
    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    Use
    Synthetic Biology
    Tags
    monomeric Enhanced Green Fluorescent Protein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    165097-plasmid-map-sequence-id-324525

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  7. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

    Plasmid
    #168491
    Purpose
    Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168491-plasmid-map-sequence-id-332602

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  8. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  9. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  11. sfGFP-Cx43K258stop

    Plasmid
    #69025
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    super folder GFP
    Expression
    Mammalian
    Mutation
    non-monomerized sfGFP fused in frame to N-terminu…
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69025-plasmid-map-sequence-id-127523

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  17. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid
    #114133
    Purpose
    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114133-plasmid-map-sequence-id-225608

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  19. pKK-TEV-EGFP

    Plasmid
    #105793
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105793-plasmid-map-sequence-id-203384

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  20. pKK-EGFP-TEV

    Plasmid
    #105776
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105776-plasmid-map-sequence-id-203365

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  22. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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  23. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid
    #174629
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174629-plasmid-map-sequence-id-346983

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Showing: 121 - 150 of 322 results