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Plasmid#222869PurposeExpresses mTagBFP2 along with an shRNA against rat PGK1DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
Plasmid#191527PurposeDonor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG) + 3xFLAG_TwinStrepExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry
Plasmid#164917PurposeDivergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizerDepositorInsertP(PGK1)-citrine and P(HIS4)-mCherry
UseTagsExpressionYeastMutationPromoterPGK1 (yeast) and HIS4 (yeast)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPGK1 (yeast)Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)
Plasmid#115429PurposeSelection for in-frame fusion expression constructsDepositorTypeEmpty backboneUseTagsT2A-SpHIS5 and λNExpressionYeastMutationPromoterPGK1Available sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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N1199 launching
Plasmid#160386PurposeExpression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequenceDepositorInsertN1199
UseTagsExpressionYeastMutationPromoterPGK1Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only