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Showing: 9961 - 9980 of 24295 results

  1. pET28a-LSSmGFP-TEV-SAV

    Plasmid
    #191200
    Purpose
    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191200-plasmid-map-sequence-id-376986

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  2. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  10. DG SaCas9 GFP V3

    Plasmid
    #226961
    Purpose
    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226961-plasmid-map-sequence-id-450824

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  11. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-450823

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  12. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

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  13. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  14. DG SaCas9 puro V3

    Plasmid
    #226960
    Purpose
    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226960-plasmid-map-sequence-id-450810

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  15. pUB_SM-FLuc0_EMCV-Ren

    Plasmid
    #210590
    Purpose
    Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-nonoptimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210590-plasmid-map-sequence-id-416804

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  16. pUB_SM-FLuc100_EMCV-Ren

    Plasmid
    #210591
    Purpose
    Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-optimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210591-plasmid-map-sequence-id-416807

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Showing: 9961 - 9980 of 24295 results