Skip to main content
Addgene
Showing: 9961 - 9980 of 20487 results
  1. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid
    #189636
    Purpose
    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries
    Depositor
    Insert
    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. qTAG-N-Puro-mNeon-ACTB

    Plasmid
    #207752
    Purpose
    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Insert
    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. qTAG-N-Blast-mNeon-ACTB

    Plasmid
    #207751
    Purpose
    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Insert
    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pCK340

    Plasmid
    #192639
    Purpose
    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Inserts
    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpG has se…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 9961 - 9980 of 20487 results