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Plasmid#185896PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER4R
Plasmid#185884PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-LifeAct-jGCaMP8f-WPRE
Plasmid#186038PurposeAAV-mediated expression of LifeAct-jGCaMP8f under the synapsin promoterDepositorInsertLifeAct-jGCaMP8f
UseAAVTagsLifeAct-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-LifeAct-jGCaMP8s-WPRE
Plasmid#186040PurposeAAV-mediated expression of LifeAct-jGCaMP8s under the synapsin promoterDepositorInsertLifeAct-jGCaMP8s
UseAAVTagsLifeAct-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-DIPP1-HA
Plasmid#183946PurposeExpresses human HA-tagged DIPP1 from yeast GPD promoterDepositorInsertDIPP1 (NUDT3 Human)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgNT-hU6-sgLkb1_1st
Plasmid#177221PurposeExpresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgNT1/sgLkb1_1st
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-DIPP3A-HA
Plasmid#183948PurposeExpresses human HA-tagged DIPP3A from yeast GPD promoterDepositorInsertDIPP3A (NUDT10 Human)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-DIPP2-HA
Plasmid#183947PurposeExpresses human HA-tagged DIPP2 from yeast GPD promoterDepositorInsertDIPP2 (NUDT4 Human)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN23-LIC-FLAG-STREP
Plasmid#182508PurposepET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREPDepositorTypeEmpty backboneUseTagsSTREP-FLAG-TEVExpressionBacterialMutationPromoterT7Available sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shTHUMPD3-Blast
Plasmid#135690PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selectionDepositorInsertshRosa26
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shTHUMPD3-Puro
Plasmid#135691PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selectionDepositorInsertshRosa26
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Blast
Plasmid#135688PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Mospd1
Plasmid#175132PurposeLentiviral expression of V5-tagged mouse Mospd1DepositorInsertMospd1 (Mospd1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tyw1-V5
Plasmid#175131PurposeLentiviral expression of mouse Tyw1-V5DepositorInsertTyw1 (Tyw1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgNT-hU6-sgLkb1_2nd
Plasmid#177223PurposeExpresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgNT1/sgLkb1_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgAmpk1-hU6-sgAmpk2
Plasmid#177224PurposeExpresses Ampk1 and Ampk2 targeting gRNAs and Cre-recombinaseDepositorInsertsgPrkaa1/sgPrkaa2
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgLkb1_2nd-hU6-sgNT
Plasmid#177222PurposeExpresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgLkb1_2nd/sgNT1
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgSik3_2nd
Plasmid#177219PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgSik3_1st
Plasmid#177218PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_1st
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only