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Plasmid#167595PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.DepositorInsertFepA
UseTagsExpressionBacterialMutationHis6 tag inserted after residue 393 in the mature…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168168PurposeExpression of YFP-CaCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
UseTagsYFPExpressionWormMutationE497K and C566DPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168171PurposeExpression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
UseTagsYFPExpressionWormMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2a
Plasmid#164963PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2a (kcnt2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfPV-1.8k-Venus-WPRE
Plasmid#156398PurposeExpresses Venus under PV gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSP-0.8k-Venus-WPRE
Plasmid#156397PurposeExpresses Venus under SP gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSP-1.7k-Venus-WPRE
Plasmid#156396PurposeExpresses Venus under SP gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSERT-0.5k-Venus-WPRE
Plasmid#156395PurposeExpresses Venus under SERT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfvAChT-1.1k-Venus-WPRE
Plasmid#156390PurposeExpresses Venus under vAChT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfCCK-3.9k-Venus-WPRE
Plasmid#156391PurposeExpresses Venus under CCK gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPS1-Venus
Plasmid#133118PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.DepositorInsertpYPS1-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMK2-Venus
Plasmid#133119PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.DepositorInsertpCMK2-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGYP7-Venus
Plasmid#133120PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.DepositorInsertpGYP7-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.1
Plasmid#149430PurposeminiTdg gene, catalytic mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationN151A catalytic mutant, SNPs of Tdg gene in OLA/1…PromoterTdg promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-Laforin
Plasmid#157989PurposeExpresses Laforin in E.coli cellDepositorInsertLaforin (EPM2A Human)
UseTagsHis TagExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
D38_Ld_341960
Plasmid#135852PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertthioredoxin, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only