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pmiRGLO-ADGRL1-201

Plasmid

#117336

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR ADGRL1-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-D167N-IRES-eGFP

Plasmid

#28098

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains D167N mutation in Cdk9

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-LpIA(wild-type)

Plasmid

#61821

Purpose

Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Use

Tags

6XHis, EGFP, and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

Plasmid

#186987

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, Gluc crRNA guide

Use

AAV

Tags

Expression

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Cep57

Plasmid

#136867

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep57 (CEP57 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO CBX1-APEX2

Plasmid

#129642

Purpose

Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

CBX1 (CBX1 Human)

Use

Tags

1xFLAG and APEX2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF63-2xCSTREP

Plasmid

#136223

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF63

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GW1-mito-mCherry-roGFP2

Plasmid

#107220

Purpose

Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mCherry-roGFP2

Use

Tags

4xCox8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K8a-2xCSTREP

Plasmid

#136158

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8a

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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myc-Atg14 S29D

Plasmid

#86757

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S29D

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-human TCR zeta/2470

Plasmid

#11507

Depositor

Allan Weissman

Article

Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

Insert

TCR zeta (CD247 Human)

Use

General cloning

Tags

Expression

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF39-2xCSTREP

Plasmid

#136200

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF39

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#2-mCherry

Plasmid

#164099

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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