Addgene

Showing: 9941 - 9960 of 52614 results

Results per page:

Sort results by:

pMAL-C2X dAmp/Kana+-2AFP

Plasmid

#154358

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

Plasmid

#133796

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1 (truncated C-terminal domain only)

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

C-terminal domain only

Promoter

CMV and T7

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

Plasmid

#133798

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T114A/F115A)

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T114A/F115A

Promoter

CMV and T7

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-H2-Ld-VN

Plasmid

#135520

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKI-cjACTB-EGFP-3'short

Plasmid

#129749

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
miR290-SE-RGM-tdTomato targeting vector

Plasmid

#133047

Purpose

Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKI-cjACTB-EGFP-5'short

Plasmid

#129748

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKI-cjACTB-EGFP

Plasmid

#129747

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/myc/ER-αT2ib

Plasmid

#110769

Purpose

Expresses ER intrabody against mouse and human TLR2

Depositor

Thomas Böldicke

Article

Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

Insert

intrabody against mouse and human TLR2

Use

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hsa-cdk2 3'UTR sicheck2 mut

Plasmid

#121124

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)

Use

Tags

Expression

Bacterial

Mutation

miR-199 seed binding site mutated

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-mcherry-2a-CDK2

Plasmid

#121131

Purpose

neutrophil specific expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-WT CDK2 (cdk2 Zebrafish)

Use

Tags

mcherry-2a

Expression

Bacterial

Mutation

Promoter

neutrophil specific lyzC

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-mcherry-2a-CDK2-D145N

Plasmid

#121132

Purpose

neutrophil specific expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-D145N CDK2 DN (cdk2 Zebrafish)

Use

Tags

mcherry-2a

Expression

Bacterial

Mutation

D145N point mutation DN

Promoter

neutrophil specific lyzC

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
dre-cdk2 3'UTR sicheck2 199 mut

Plasmid

#121122

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

miR-199 seed binding site mutated

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
dre-cdk2 3'UTR sicheck2

Plasmid

#121123

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-cdk2 3'UTR (cdk2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ150-x3.7

Plasmid

#124308

Purpose

Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9-x3.7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Designed HEL-binding scFv D44.1des

Plasmid

#111719

Purpose

Yeast Surface Display of D44.1des

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1 des

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB61 - pL0_V2 (CDS1)

Plasmid

#123183

Purpose

Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ151D10A-x3.7

Plasmid

#124309

Purpose

Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-x3.7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV CMV-driven AcrIIA4-2xmiR-1 target sites

Plasmid

#120299

Purpose

AAV vector for expression of AcrIIA4 with two miR-1 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4-2xmiR-1 binding sites

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge