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Plasmid#140227PurposeHypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.DepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseLentiviralTagsExpressionMammalianMutationPromoterHRE (Hypoxia inducible)Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4-ires-blast
Plasmid#167829PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EPHB2 gRNA (BRDN0001146273)
Plasmid#77545Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB2DepositorInsertEPHB2 (EPHB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1 gRNA (BRDN0001145303)
Plasmid#77733Purpose3rd generation lentiviral gRNA plasmid targeting human ABL1DepositorInsertABL1 (ABL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUp95bGW (B6)
Plasmid#74015Purposelentiviral expression of shRNA resistant Psd95-beta-EGFP fusionDepositorInsertPsd95 beta isoform
UseLentiviralTagsEGFPExpressionMammalianMutationshRNA resistant Psd95PromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ERBB4 (E542K)
Plasmid#29539DepositorInsertERBB4 (ERBB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_Balbc
Plasmid#170301PurposeA knock-out vector for the mouse Ptgs2 in Balb/cDepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SUV39h1 ts1
Plasmid#115880PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK477
Plasmid#138005Purpose3xMYC-SIX2 expression. Vector also expresses GFP under control of hUBC promoterDepositorInsert3xMYC tagged SIX2 (SIX2 Human)
UseLentiviralTags3xMYCExpressionMutationNonePromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ERBB3 gRNA (BRDN0001147088)
Plasmid#77481Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB3DepositorInsertERBB3 (ERBB3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
YES1 (T348I) in pLX303
Plasmid#42564DepositorInsertYES1 (YES1 Human)
UseLentiviralTagsExpressionMammalianMutationT348IPromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRRL WS1.6 GFP
Plasmid#36249DepositorInsertGFP (eGFP )
UseLentiviralTagsExpressionMutationPromoterWAS (human 1.6 kb)Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLN397 (FuGW-S(E2F1)p-Sponge7)
Plasmid#105181PurposeModule 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(E2F1)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)
Plasmid#105192PurposeModule 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)DepositorInsertS(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
Plasmid#20295PurposeLentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to ThreoninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-TbID-LBR
Plasmid#175100PurposeLentiviral expression of V5-TbID-tagged mouse LbrDepositorInsertLbr (Lbr Mouse)
UseLentiviralTagsV5-TbIDExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Arch-EEN
Plasmid#45189PurposeArch voltage indicator with double mutations D95N-D106E (Arch-EEN), with faster kinetics and greater fluorescence dynamic range than Arch-D95NDepositorInsertenhanced Archaerhodopsin-3
UseLentiviralTagseYFPExpressionMammalianMutationD95N, D106EPromoterCamKIIaAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode
Plasmid#170225PurposeBarcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertER (ESR1 Human)
UseLentiviralTagsV5ExpressionMutationY537SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_V5.AU1.FLAG_NGFR
Plasmid#158240PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsV5.AU1.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only