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AAV-AID-N51A-CBE N

Plasmid

#227421

Purpose

expresses the N-terminal of AID-N51A-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-AID-N51A-CBE N-terminal (AICDA Human)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(3mut)-mGreenLantern

Plasmid

#219662

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1

Plasmid

#223315

Purpose

Lentiviral vector expressing human PNPT1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(WT)

Plasmid

#224283

Purpose

Cell transfection and expression of K-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-scFVDnmt3AL_bGHpA

Plasmid

#177347

Purpose

AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)

Use

AAV

Tags

C-terminal domain of mouse Dnmt3l (194–415 aa), P…

Expression

Mammalian

Mutation

598–908 aa of mouse Dnmt3a

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-scFVtet1_bGHpA

Plasmid

#177351

Purpose

AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

N terminal domain of human Tet1

Promoter

CMV promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-Antares

Plasmid

#208852

Purpose

Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

Antares

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-Nipah V

Plasmid

#174228

Purpose

Expresses Nipah virus V

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

Nipah V

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RFX5

Plasmid

#222545

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RFX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RFX5 (RFX5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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