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AAV-NG-CBE C

Plasmid

#227422

Purpose

expresses the C-terminal of AAV-NG-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-NG-CBE C-terminal

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p36.pA.NGFR.mCMV.hPGK.mGLuc(flag).WPRE[sequenced]

Plasmid

#226731

Purpose

backbone vector

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Use

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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p34.pA.NGFR.mCMV.hPGK.mSrtA(Ge)TM.WPRE[sequenced]

Plasmid

#226759

Purpose

sortase A (GE) - TM in p36

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(5mut)-mGreenLantern

Plasmid

#219661

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Lrrc59

Plasmid

#226565

Purpose

Encodes Lrrc59 protein laebeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Lrrc59

Plasmid

#226564

Purpose

Encodes Lrrc59 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TurboID-CRaf

Plasmid

#226168

Purpose

Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-CRaf (RAF1 Human)

Use

Lentiviral

Tags

EGFP-IRES-BlastR and Myc-TurboID

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AID-N51A-NG-CBE

Plasmid

#227417

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-N51A-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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