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Plasmid#160408PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn systemDepositorInsertpDONR221 hyCal2
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH54_pE12_hyCal7
Plasmid#160409PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn systemDepositorInsertpDONR221 hyCal7
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH55_pE12_hyCal9
Plasmid#160410PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn systemDepositorInsertpDONR221 hyCal9
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH119_pCMV_HyPep-emGFP
Plasmid#160417PurposeCMV promoter driving GFP labeled version of HyPepDepositorInsertpEZY3 CMV-synthNP-emGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH120_pE12_HyPep_emGFP
Plasmid#160406PurposeGateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySynDepositorInsertpDONR221 HyPep emGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH161_pE12_HyPep_mRuby
Plasmid#160407PurposeGateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySynDepositorInsertpDONR221 HyPep mRuby
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc318
Plasmid#161941PurposeCarries Golden Gate substitution insert for ER-LBD domain-insertion mappingDepositorInsertpSB1A3-BbsI-G-ER-LBD-S-SapI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2-YFP
Plasmid#84544PurposeHybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFPDepositorInsertT2U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U3-YFP
Plasmid#84547PurposeHybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFPDepositorInsertT2U3-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T4-YFP
Plasmid#84548PurposeHybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFPDepositorInsertU2T4-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2T2-YFP
Plasmid#84549PurposeHybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertT2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T2U2T2-YFP
Plasmid#84550PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T4U2-YFP
Plasmid#84551PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFPDepositorInsertU2T4U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2T2U2-YFP
Plasmid#84552PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFPDepositorInsertT2U2T2U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U4T2-YFP
Plasmid#84553PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFPDepositorInsertT2U4T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T2-YFP
Plasmid#84543PurposeHybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only