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pUC57-Kan_BR32_APikL2

Plasmid

#123649

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL3

Plasmid

#123650

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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PKAcs-KR6X-GFP

Plasmid

#108585

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with structured linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PKAcs-L10X-GFP

Plasmid

#108587

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with structured linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz3

Plasmid

#74362

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

eashirt zinc finger family member 3 (Tshz3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Msx2

Plasmid

#84314

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx2 (Msx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBS RAI14

Plasmid

#19204

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Retinoic acid induced 14 (RAI14 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS WSB2

Plasmid

#19190

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

WSB2 (WSB2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus

Plasmid

#212665

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hCDC6p

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Noodles Plasmid

Plasmid

#212613

Purpose

An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

Depositor

Ilya Vinnikov

Article

Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

Type

Empty backbone

Use

CRISPR and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-AqpZ-ALFA

Plasmid

#212310

Purpose

Expression of AqpZ with ALFA tag

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

Aquaporin Z

Use

Tags

ALFA tag and Strep-Tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pTGL3

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pPEX3

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHF distal site is removed

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCB40

Plasmid

#205136

Purpose

Integrating expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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