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Plasmid#75756Purpose3rd generation lentiviral gRNA plasmid targeting human SIK3DepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIK3 gRNA (BRDN0001149197)
Plasmid#75757Purpose3rd generation lentiviral gRNA plasmid targeting human SIK3DepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF16_P2A_Hygro_Barcode
Plasmid#120502PurposeBarcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF16 (KLF16 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_sh_ex15
Plasmid#35166DepositorInsertFoxp1 (Foxp1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E4-T2A-Puro
Plasmid#190813PurposeExpress APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E3-T2A-Puro
Plasmid#190812PurposeExpress APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E2-T2A-Puro
Plasmid#190811PurposeExpress APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorInsertTmem53 (Tmem53 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.13.EFS-NS.H2B-RFP
Plasmid#170388PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-PARK7WT-VA
Plasmid#115182PurposeLentiviral transduction and expression of PARK7WT into any mammalian cellDepositorInsertPARK7WT (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDGFRA gRNA (BRDN0001148373)
Plasmid#75493Purpose3rd generation lentiviral gRNA plasmid targeting human PDGFRADepositorInsertPDGFRA (PDGFRA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone32 (Opti-SpCas9)
Plasmid#131736PurposeExpresses Opti-SpCas9 in mammalian cellsDepositorInsertOpti-SpCas9
UseLentiviralTagsFlagExpressionMutationR661A+K1003HPromoterEFSAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4(TA)-ires-puro
Plasmid#167830PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV CDHblast Rev(V16D)-EGFP-V5
Plasmid#175165PurposeLentiviral expression of Rev(V16D)-EGFP-V5DepositorInsertrev
UseLentiviralTagsV5ExpressionMammalianMutationV16DPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-lincRNA-RoR-sh1 (Linc-sh1)
Plasmid#45764DepositorInsertlincRNA-RoR-shRNA1 (LINC-ROR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_GAL4UAS-Luciferase reporter
Plasmid#64158PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA2 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKDR-Lenti-CBh.6-SOD1
Plasmid#188460PurposeFor knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMutationPromoterCBhAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Crimson/P2A-NLS/HA-HumBeta (pSLIK2)
Plasmid#113857PurposeFor Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase geneDepositorInsertHumanized Beta
UseLentiviralTagsP2A/CrimsonExpressionMutation"humanized" Beta genePromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLK1 gRNA (BRDN0001147678)
Plasmid#76460Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only