-
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorInsertUtrophin (UTRN Human)
UseTagsEBFP2ExpressionMammalianMutationsee depositor comments belowPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-838;+80pCalb2
Plasmid#66750Purposeluciferase reporter for Calb2 promoter (-838 to +80, WT)DepositorInsertCalb2 promoter (-838bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
D998-EGFR-ET1
Plasmid#44186DepositorInsertD998-EGFR-ET1 (EGFR Human)
UseTags3C cleavge site, protein C peptide tag, his6, SBP…ExpressionMammalianMutationEGFR amino acids 1−998PromoterCVMAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 TO FRT Myc ArB K106R
Plasmid#59807PurposeAllows the integration of Myc ArB K106R in the genome and Tet-inducible expressionDepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationK106R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL2
Plasmid#89295PurposeFor expression of the mammalian Atg8 protein GABARAPL2 without a tagDepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-RanV19 E1RA2
Plasmid#118317PurposeExpression of human RanDepositorUseTagsGFPExpressionMammalianMutationin-frame insertion of RanV19 into GFPEpac1RA2 and…PromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-ENLYFQC-PNT(46-138)-His6
Plasmid#42919DepositorInsertEts-1 PNT domain (46-138) (Ets1 Mouse)
UseTags6xHis, GST, and TEV protease cleavage siteExpressionBacterialMutationContains the PNT domain (Serine 46 through Lysine…PromoterPtacAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-BAIAP2 S366A
Plasmid#31676DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
UseTagsGSTExpressionBacterialMutationchanged Serine 366 to AlaninePromoterAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorInsertPHD2 (EGLN1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-KRas-Q61L
Plasmid#214300PurposeExpress constitutively active KRas fused with MBPDepositorInsertConstitutively active KRas (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-KRas-S17N
Plasmid#214301PurposeExpress dominant negative KRas fused with MBPDepositorInsertDominant negative KRas (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-S17NPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFBgRNA9
Plasmid#196108PurposeContains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107DepositorInsertSAFB (Safb Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
tetO-IRX5
Plasmid#170693Purposedoxycycline-inducible overexpression of IRX5DepositorInsertIRX5 (IRX5 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2Y pc644
Plasmid#167565PurposeThe complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)DepositorInsertMethyl-CpG binding protein 2 (Mecp2 Rat)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_KLF4
Plasmid#140104PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 L211P
Plasmid#124487Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged leucine 211 to prolinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 R312H
Plasmid#124488Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged arginine 312 to histidinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only