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Plasmid#72359PurposeDonor vector for 3' FLAG tag of human ZNF146DepositorInsertZNF146 Homology Arms (ZNF146 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS2-his-24::tagBFP
Plasmid#124320PurposeExpressing Nuclear Localized tagBFPDepositorInserttagBFP (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP4xN_ccdB
Plasmid#79022PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6-MBP TEV Ade S. cerevisiae expression vector (12ADE-C)
Plasmid#48299DepositorTypeEmpty backboneUseNaTagsHis6 and MBPExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9a)
Plasmid#163419PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shRen-Blast
Plasmid#135684PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorInsertSSRP1 Homology Arms (SSRP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PG1424PGA
Plasmid#74652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPG1424PGA
UseTagsHis-6-TEVExpressionBacterialMutationPromoterT7Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_100_CA_repeat
Plasmid#65867PurposeCarries CA repeats of 50-100 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50-100 nucleotides in lngth
UseTagsExpressionMutationPromoterT7Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
UC20m
Plasmid#121040PurposeMoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).DepositorInsertgN2 Cas9 gRNA UC part
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC49
Plasmid#104823PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma17g11235
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TARS_pNIC-Bio3
Plasmid#153057PurposeExpression construct for producing Avi tagged Threonyl-tRNA synthetaseDepositorInsertTARS (TARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-D149A
Plasmid#29418DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationAsp149AlaPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
C71m
Plasmid#120983PurposeMoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertCinR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-Epo
Plasmid#114290PurposeTET-inducible expression of ErythropoietinDepositorInsertErythropoietin (Epo Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PARP14A
Plasmid#42487PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPARP14A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEos-A69V-C1
Plasmid#54647PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos A69VExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTMR6A
Plasmid#42483PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMTMR6A
UseTagsHis6ExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only