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pcDNA3.1(+) CircRNA Mini Vector

Plasmid

#60648

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCitrine

Plasmid

#188386

Purpose

Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Gal4UAS_tBFP_PGK_mCitrine

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pVoPo-04

Plasmid

#187839

Purpose

Suicide vector to generate markerless gene deletions in Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk4-HA

Plasmid

#1876

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP14

Plasmid

#22569

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP14 (USP14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pJMP1039

Plasmid

#119239

Purpose

expresses Tn7 transposase

Depositor

Oren Rosenberg, Carol Gross, Jason Peters

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

tnsABCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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