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Plasmid#66924Purpose3rd generation lentiviral Expression of TRAF2DepositorInsertTRAF2 (TRAF2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)
Plasmid#48296DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL1
Plasmid#101910PurposeExpresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7+LacOAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAmKate
Plasmid#32691DepositorInsertPAmKate
UseTagsExpressionBacterialMutationF84W/S148N/S165G compared to mKate (but numbering…PromoteraraBAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
SETD1A
Plasmid#32868PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD1A (SETD1A Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
piB-GFP
Plasmid#13844DepositorInsertTATA-GFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_SDHA
Plasmid#177980Purposelentiviral vector expressing Cas9 and a sgRNA targeting SDHADepositorInsertsgRNA targeting SDHA (SDHA Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse Clock delta 19
Plasmid#177312Purposeexpression of mutant mouse clockDepositorInsertmouse clock delta 19
UseTagsExpressionMammalianMutationdeletion from original mClock gene (514-564aa)PromoterCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only