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Plasmid#192126PurposeExpresses Hsp2Cas9DepositorInsertHsp2Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Hsp1Cas9
Plasmid#192125PurposeExpresses Hsp1Cas9DepositorInsertHsp1Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Cj-sgRNA-SV40-puro
Plasmid#192128PurposeExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCj-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxD
Plasmid#188308PurposeExpression of LuxD via a T7 promoter in TXTLDepositorInsertluxD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mGFP-MutCREB(S133A)
Plasmid#194642PurposeExpresses the fusion of monomeric GFP and mutant CREB(S133A) in a Cre-dependent mannerDepositorInsertMonomeric GFP and mutant CREB(S133A) fusion
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterTag
Plasmid#186910PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5-420
Plasmid#192993PurposePlasmid that encodes for Hydra Sp5DepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-Luz
Plasmid#188304PurposeExpression of Luz via a T7 promoter in TXTLDepositorInsertluz
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-LexAOx4-Pmin-mCherry
Plasmid#192633PurposeLentiviral vector for inducible mCherry expression.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal promoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuzGFPHis
Plasmid#188315PurposeExpression of Luz-eGFP via a T7 promoter in TXTLDepositorInsertLuz-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-Fre
Plasmid#188310PurposeExpression of Fre via a T7 promoter in TXTLDepositorInsertfre
UseSynthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxC
Plasmid#188307PurposeExpression of LuxC via a T7 promoter in TXTLDepositorInsertluxC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-H3H
Plasmid#188303PurposeExpression of H3H via a T7 promoter in TXTLDepositorInserth3h
UseSynthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPLuz
Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxE
Plasmid#188309PurposeExpression of LuxE via a T7 promoter in TXTLDepositorInsertluxE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_E2At(2nd)
Plasmid#183085Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2At(2nd)
Plasmid#183086Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2At(2nd)
Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only