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Plasmid#174871PurposeCRISPR vector for generating Nanog STREAMING-tag KI cellDepositorInsertsgRNA for mouse Nanog (Nanog Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKalpha2 M93G
Plasmid#31655DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationchanged Methionine 93 to GlycinePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_HSP7C
Plasmid#111667PurposeMAC-tagged gene expressionDepositorInsertHSP7C (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p 3 perfect copies.
Plasmid#82439PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copiesDepositorInserthsa-miR-27a-3p (MIR27A Human)
UseTagsExpressionMutationThree copies hsa-miR-27a-3p Complementary region …PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
200 bp Axin2 mutant
Plasmid#25704DepositorInsertAxin 2 promoter (AXIN2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTCF DNA binding site at bp -108 to -102 mutated …PromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_A263insGG
Plasmid#31103DepositorInsertHNF1B (HNF1B Human)
UseFlp/frtTagsmycExpressionMammalianMutationmutation A263insGGPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
ARB365
Plasmid#124048PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_P328L329del
Plasmid#31102DepositorInsertHNF1B (HNF1B Human)
UseFlp/frtTagsmycExpressionMammalianMutationmutation P328L329delCCTCTPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
UseTagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-p38 β/MAPK11 5'-UTR
Plasmid#115357Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNADepositorInsertp38 β/MAPK11 5'-UTR (MAPK11 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMextli
Plasmid#79262PurposeExpresses EGFP-tagged DmMextli in S2 cellsDepositorInsertDmMextli (mxt Fly, NM_164598)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1:mTRESK(S116C)
Plasmid#224772PurposeIn vitro transcription for Oocyte expression of mTRESK(S116C)DepositorInsertPotassium channel subfamily member 18 (Kcnk18 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS116CPromoterT7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C)
Plasmid#224744PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131A)
Plasmid#224745PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G171F)
Plasmid#224747PurposeIn vitro transcription for Oocyte expression of mTREK-1(G171F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationG171FPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(A286F)
Plasmid#224746PurposeIn vitro transcription for Oocyte expression of mTREK-1(A286F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationA286FPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-PRF1-T2A-cDNA
Plasmid#209076PurposeTargeting vector for the human PRF1 locus to replace exon 3 with repaired cDNADepositorInsertPRF1-T2A-cDNA HDRT (PRF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HaloTag-KRas4B(CT)
Plasmid#214276PurposeMammalian expression of HaloTag fused to the C-terminal tail of KRas4BDepositorArticleInsertHaloTag7-KRas4B(174-188) (KRAS Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFBgRNA8
Plasmid#196103PurposeContains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108DepositorInsertSAFB (Safb Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only