Addgene

Showing: 961 - 980 of 3121 results

Results per page:

Sort results by:

pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Multisite gateway entry clone

Tags

3XFLAG

Expression

Mutation

S127A

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP scFv [N86/44]

Plasmid

#206743

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5-LAP-MPS1-KD

Plasmid

#114045

Purpose

Expression of exogenous LAP tagged MPS1 Kinase-dead

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-KD (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

D664A kinase dead

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.A184V

Plasmid

#106187

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

Plasmid

#85111

Purpose

expression of recombinant human mature (delta19) ETFalpha wt without tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta19) ETFalpha (ETFA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTTH+mEGFP-LANA-H1.4CTD

Plasmid

#157802

Purpose

Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA-H1.4CTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHMP2A-NS3-blue

Plasmid

#223532

Purpose

Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP2A (CHMP2A Human)

Use

Tags

NS3 Hepatitis C protease and mTurquoise

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHMP2A-NS3-Green

Plasmid

#223440

Purpose

Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP2A (CHMP2A Human)

Use

Tags

NS3 Hepatitis C protease and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHMP3-NS3-Blue

Plasmid

#223533

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Use

Tags

NS3 Hepatitis C protease and mTurquoise

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHMP3-NS3-Green

Plasmid

#223527

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Use

Tags

NS3 Hepatitis C protease and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv2.1 K+ channel scFv [D4/154]

Plasmid

#206787

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma D4/154 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel recombinant scFV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmirGLO-3'UTR mouse Il13 5xBoxB

Plasmid

#207128

Purpose

Tethered luciferase reporter for mouse Il13 BoxB

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Tags

5xBoxB

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KCC2 scFv [N1/12]

Plasmid

#190520

Purpose

Mammalian Expression of KCC2 scFV. Derived from hybridoma N1/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

KCC2 (Rattus norvegicus) recombinant scFV (Slc12a5 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Use

Tags

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EB3-mNeonGreen

Plasmid

#191761

Purpose

Expression of mNeonGreen fused to EB3

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

EB3-mNeonGreen (MAPRE3 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-3XHA-p53

Plasmid

#196267

Purpose

Mammalian expression of 3xHA tagged wild-type p53

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 (Trp53 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge