We narrowed to 5,041 results for: golden gate
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Plasmid#121019PurposeMoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD20
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U7m
Plasmid#121020PurposeMoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD16
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U8m
Plasmid#121021PurposeMoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD24
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C92m
Plasmid#121000PurposeMoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).DepositorInsertsfCFPpdt3 (Chen,Bennett)
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C101m
Plasmid#121005PurposeMoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).DepositorInsertsfCFP with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C63m
Plasmid#120979PurposeMoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlacI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P60m
Plasmid#120952PurposeMoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved pSal promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertpSal Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C20m
Plasmid#120961PurposeMoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)DepositorInsertsfYFP-ssrA (has BbsI site)
UseSynthetic BiologyTagsssrA degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P30m
Plasmid#120942PurposeMoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpL-lacO1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U24m
Plasmid#121026PurposeMoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).DepositorInsertRiboJ + BCD2
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C67m
Plasmid#120981PurposeMoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBxb1 integrase - mScarlet-i - LAA tag
UseSynthetic BiologyTagsssrA degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C71m
Plasmid#120983PurposeMoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertCinR Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_3_XcmI-LacZ
Plasmid#49298PurposeGolden Gate entry vector at position 3, LacZDepositorTypeEmpty backboneUseCloning vectorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
U9m
Plasmid#121022PurposeMoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD22
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T2m
Plasmid#121029PurposeMoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertECK120033736 (164.6x Voigt terminator), short attachment
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T13m
Plasmid#121032PurposeMoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertECK120033737 (second strongest natural Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T14m
Plasmid#121033PurposeMoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S1P13 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only