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Showing: 961 - 980 of 27415 results

  1. pUC57-4eCOL2A1

    Plasmid
    #97211
    Purpose
    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Vector is designed for cloning and generation of …
    Tags
    Expression
    Mutation
    Promoter
    COL2A1 regulatory elements
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97211-plasmid-map-sequence-id-181460

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  3. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  5. Linked Free NES

    Plasmid
    #182485
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182485-plasmid-map-sequence-id-360436

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  6. Linked Free PcPTS

    Plasmid
    #182486
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-PTS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182486-plasmid-map-sequence-id-360437

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  7. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182487-plasmid-map-sequence-id-360431

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  11. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

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  15. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  18. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  19. pACB120

    Plasmid
    #222213
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(K10N, S11R, W15C, G76V, G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Contains five amino acid mutations: K10N, S11R, W…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222213-plasmid-map-sequence-id-443111

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  20. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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Showing: 961 - 980 of 27415 results