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Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1163A
Plasmid#194001PurposepBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.DepositorInsertUniq-CasRx (Codon optimized)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-hMIGA2 (170-593)
Plasmid#192833PurposeExpress codon-optimized human MIGA2 fragment 170-593 in Expi293 cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG101-W110D/F112D
Plasmid#188106PurposeExpresses human ATG101 HORMA W110D/F112D mutant in mammalian cellsDepositorInsertATG101 (ATG101 Human)
UseTagsGST tagExpressionMammalianMutationcodon-optimized ATG101-W110D/F112DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL H163A H258A 3xFLAG-V5
Plasmid#174131PurposeExpresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimized, H163A, H258APromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL H258A 3xFlag-V5
Plasmid#174130PurposeExpresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimized, H258APromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-Nprot_GC3opt
Plasmid#157730Purposemammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertNprot_GC3opt (N SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8_GC3opt
Plasmid#157697Purposemammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt
Plasmid#157700Purposemammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp8_GC3opt
Plasmid#157698Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
INCTbiosyn-pUB-HspINT2
Plasmid#127512PurposePlasmid encodes H. sapiens codon optimized Integrase 2.DepositorInsertIntegrase 2 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT4
Plasmid#127513PurposePlasmid encodes H. sapiens codon optimized Integrase 4.DepositorInsertIntegrase 4 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT5
Plasmid#127514PurposePlasmid encodes H. sapiens codon optimized Integrase 5.DepositorInsertIntegrase 5 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT9
Plasmid#127516PurposePlasmid encodes H. sapiens codon optimized Integrase 9.DepositorInsertIntegrase 9 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT13
Plasmid#127517PurposePlasmid encodes H. sapiens codon optimized Integrase 13.DepositorInsertIntegrase 13 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)
Plasmid#133796PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1 (truncated C-terminal domain only)
UseTagsNLS(SV40)ExpressionMammalianMutationC-terminal domain onlyPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
Plasmid#133798PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T114A/F115A)
UseTagsNLS(SV40)ExpressionMammalianMutationT114A/F115APromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL33
Plasmid#135103PurposeCodon-optimized HSV-1 UL33 with N-terminal HAStrep tagDepositorInsertHSV-1 UL33 codon optimized
UseTagsHA-Strep-StrepExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL50-CStHA
Plasmid#135105PurposeCodon-optimized HSV-1 UL50 with C-terminal StrepHA tagDepositorInsertHSV-1 UL50 codon optimized
UseTagsStrep-Strep-HAExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL33-CStHA
Plasmid#135106PurposeCodon-optimized HSV-1 UL33 with C-terminal StrepHA tagDepositorInsertHSV-1 UL33 codon optimized
UseTagsStrep-Strep-HAExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only