Addgene

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pAc2_Cas7

Plasmid

#178739

Purpose

Expression of A. chroococcum type I-E #2 cas7

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-Fc-His

Plasmid

#72098

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.5-Fc-His

Plasmid

#72102

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-AP-His

Plasmid

#71972

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.3-Fc-His

Plasmid

#72100

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.3 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.4-Fc-His

Plasmid

#72101

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.4 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.6-Fc-His

Plasmid

#72103

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.6 (Nrp2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.3-AP-His

Plasmid

#71974

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.3 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.6-AP-His

Plasmid

#71977

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.6 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.5-AP-His

Plasmid

#71976

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.4-AP-His

Plasmid

#71975

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.4 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 AT1AR-324delta

Plasmid

#45635

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-324delta (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

added stop codon at 324 aa

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pHDT2:GUS

Plasmid

#108444

Purpose

Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 promoter (HD2B Mustard Weed)

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

HDT2

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:HDT2-GFP

Plasmid

#108566

Purpose

Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 (HD2B Mustard Weed)

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

HDT2

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/stSpike-puro

Plasmid

#161794

Purpose

Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 secretable trimeric Spike

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/RBD-puro

Plasmid

#161793

Purpose

Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 RBD

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pXJ40-HA-Merlin I S518D

Plasmid

#19701

Depositor

Filippo Giancotti

Article

Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Insert

Merlin I (Nf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 518 to Aspartic Acid

Promoter

CMV

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge