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Showing: 961 - 980 of 2911 results

  5. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid
    #131138
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and tdTomato
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131138-plasmid-map-sequence-id-258855

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  6. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid
    #174634
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174634-plasmid-map-sequence-id-346991

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  7. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  8. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid
    #193720
    Purpose
    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193720-plasmid-map-sequence-id-383731

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  9. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid
    #193717
    Purpose
    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
    Use
    Tags
    2xmNeongreen-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193717-plasmid-map-sequence-id-383726

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  10. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid
    #131137
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131137-plasmid-map-sequence-id-258861

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Showing: 961 - 980 of 2911 results