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Plasmid#176376PurposeExpresses spCas9 and gRNA sequence targeting OTX2DepositorInsertOTX2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_TGIF2_1
Plasmid#72374PurposeEncodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFPDepositorInsertTGIF2 (TGIF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
HEY2.1.1-gDNA
Plasmid#175873PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertHEY2-Nickase1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-67
Plasmid#188671PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHCHD3-N2.1.2-gDNA
Plasmid#175858PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCHCHD3-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-213
Plasmid#219974PurposeeIF4G-213 protein purificationDepositorInserteIF4G-213 (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutation213VKLR216 to 213AAAA216PromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13209
Plasmid#219884PurposeThe base plasmid of TUNEYALI for TF19DepositorInsertContains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13229
Plasmid#219904PurposeThe base plasmid of TUNEYALI for TF39DepositorInsertContains gRNA targeting TF39 (YALI1_B16808g) and homologous arm matching TF39
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPHTF2.1.0-gDNA
Plasmid#132465PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertPHTF2 (PHTF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_2
Plasmid#72364PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-int1
Plasmid#188681Purposecontrol sgRNADepositorInsertsgPHGDH intron control (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRARB.1.0-gDNA
Plasmid#132471PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR255
Plasmid#78549PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, CMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK217
Plasmid#72471PurposeProduces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_SEO1
Plasmid#166076PurposePlasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertSEO1 (SEO1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHuR CDS
Plasmid#110426PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.DepositorInsertELAVL1 HuR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-CDK1
Plasmid#188684Purposecontrol sgRNADepositorInsertsgCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RRT8
Plasmid#166080PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.DepositorInsertPromoter of RRT8 (RRT8 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RPL22A
Plasmid#166079PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.DepositorInsertPromoter of RPL22A (RPL22A Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only