Addgene

We narrowed to 4,148 results for: 027

Showing: 961 - 980 of 4148 results

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2

Plasmid

#34691

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2 (TNKS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pIM691

Plasmid

#74641

Purpose

alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX305_3xFLAG_RELA1

Plasmid

#220324

Purpose

Expresses full length human RELA with an N-terminal 3xFLAG tag

Depositor

Jonathan Brown

Insert

RELA (RELA Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

hPGK

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pME-cas9-2A-mCherry

Plasmid

#107592

Purpose

An entry vector with cas9 -2A-mCherry

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

cas9-2A-mCherry

Use

CRISPR

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdc2-AF (718)

Plasmid

#39872

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

Cdc2 (CDK1 Human)

Use

Tags

Expression

Mammalian

Mutation

AF = T14A, Y15F

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pegRNA_LMNA-1

Plasmid

#199273

Purpose

pegRNA used to induce LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824CtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PE-Cas9

Plasmid

#174851

Purpose

Express PE-Cas9 in mammalian cells

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

PE-Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMEH373 dTomato 3x high affinity MS2 loops

Plasmid

#168221

Purpose

Mammalian expression of dTomato with 3 high affinity MS2 loops

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

dTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pC-GoldyTALEN

Plasmid

#38143

Purpose

destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

Depositor

Daniel Carlson

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Tags

AcV5 and FokI homodimer

Expression

Mammalian

Mutation

Truncate at N and C terminous

Promoter

miniCaggs

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGFP-E p27 (CC#631)

Plasmid

#15192

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only