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Plasmid#84892PurposeDONOR vector for Gateway cloning of EWSR1 c1655t No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655t No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t Stop
Plasmid#84891PurposeDONOR vector for Gateway cloning of EWSR1 c1655t StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655tPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1532c No Stop
Plasmid#84890PurposeDONOR vector for Gateway cloning of EWSR1 g1532c No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1532c No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.2
Plasmid#172320Purposeexpressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.DepositorInsertSpike of B.1.617.2 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT19R, 156G, 157-158del, L452R, T478K, D614G, P681…PromoterCMV-FAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 nonmutant
Plasmid#183813PurposeExpress CD74-ROS1 fusion (C6;R34) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/TO-3xFLAG-REV7
Plasmid#114127PurposeExpresses N-terminally tagged 3x FLAG-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTags3xFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 16, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.3-SARS2-B.1.617.1
Plasmid#172319Purposeexpressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus productionDepositorInsertSpike of B.1.617.1 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationG142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…PromoterCMVAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_Mu
Plasmid#177578Purposeexpressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus productionDepositorInsertSpike (Mu variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutationT95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_omicron_BA.2.75
Plasmid#190674Purposeexpressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus productionDepositorInsertSpike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationK147E, W152R, F157L, I210V, G257S, D339H, G446S, …PromoterCMVAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_Lambda
Plasmid#177579Purposeexpressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus productionDepositorInsertSpike (Lambda variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutation75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B
Plasmid#133308PurposeExpression of luciferase driven by eIF3B promoter regionDepositorInserteIF3B promoter (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R
Plasmid#183814PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cellsDepositorUseRetroviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032RPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/TO-eGFP-REV7
Plasmid#114128PurposeExpresses N-terminally tagged eGFP-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTagseGFPExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW-eGFP-FHA-SHLD2-m1
Plasmid#114122PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (m1 mutant) (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationW489A, F494A, W495A mutations introducedPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1P552L-CMyc
Plasmid#50736PurposeProduces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 P552L (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Pro 552 to LeuPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2086F
Plasmid#183816PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2000V
Plasmid#183815PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2000VPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Fusion + CBP TAZ1(340-439)
Plasmid#173762PurposeBacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only