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Showing: 9761 - 9780 of 11049 results

  7. ATG-1929

    Plasmid
    #108712
    Purpose
    CBR2opt in pF4Ag expression vector with T7 and CMV promoter
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    T7, CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108712-plasmid-map-sequence-id-224541

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  11. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP160
    Expression
    Mutation
    D10A;H840A nuclease-deficient
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48221-plasmid-map-sequence-id-69506

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  15. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Use
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  18. JPF0535a

    Plasmid
    #124042
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124042-plasmid-map-sequence-id-264228

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Showing: 9761 - 9780 of 11049 results