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Showing: 9741 - 9760 of 16182 results

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pDestTol2pA2-LA-nanoLuc-RA

Plasmid

#128692

Purpose

Donor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 NanoLuc

Use

Multisite-gateway assembly

Tags

NanoLuc

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 K82R

Plasmid

#45206

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 K82R

Use

Tags

Expression

Mammalian

Mutation

Plk1 K82R mutation

Promoter

T7

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pDest-305 (attR4-attR2)

Plasmid

#161895

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRGD-HmR

Plasmid

#74107

Purpose

Plasmid containing hygromycin B resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

hph5

Use

Bacterial vector based on pmb1 replicon

Tags

Expression

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pKB230

Plasmid

#170017

Purpose

Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Protegrin-1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

Plasmid

#184293

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

Depositor

Bernardo Sabatini

Inserts

sgRNA-Adra1b
sgRNA-Adra1b

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAM2314

Plasmid

#40246

Depositor

Susan Golden

Article

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

Polylinker

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pKB255

Plasmid

#170038

Purpose

Encodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Melittin

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDest-665 (attR4-attR2)

Plasmid

#161885

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-GST-CD-ATL (Cbx7)

Plasmid

#82527

Purpose

Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 7 (CBX7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1–84

Promoter

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_HD

Plasmid

#90416

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_HD

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCA1.332 pDUOTK1-L1

Plasmid

#162351

Purpose

Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

Depositor

Alistair McCormick

Article

Gale et al Front Microbiol. 2021 Jan 15;11:624011.

Inserts

eYFP
LacZ alpha
mTagBFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-tetO

Plasmid

#119000

Purpose

Repair template for the TLR reporter with 7 tet operator elements

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3-MCS

Plasmid

#26798

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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pIBG-FTet

Plasmid

#33361

Depositor

Doug Mortlock

Article

Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. E

Inserts

IRES-Bgeo
TetR cassette

Use

Mouse Targeting

Tags

FRT site

Expression

Mutation

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only