Addgene

We narrowed to 34,843 results for: lat

Showing: 9741 - 9760 of 34843 results

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p178 Grg-1L Myc His

Plasmid

#11067

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-L (Tle1 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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bactin-loxp-BFP-loxp-dsred-pBSII

Plasmid

#68972

Purpose

for transgenic animals

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Inserts

lox-BFP-loxp
dsred

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pORF1-Flag

Plasmid

#190565

Purpose

Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

ORF1 (from L1.3)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PTBP1-Halo

Plasmid

#175302

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PTBP1 (PTBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTRE-EGFP

Plasmid

#89871

Purpose

EGFP reporter vector for iTango system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCIP

Plasmid

#79009

Purpose

For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGs

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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T7-LacO-GFP_ColE1

Plasmid

#171648

Purpose

Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-GFPssrA-T7 Stop

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Use

Tags

Expression

Mutation

Promoter

no promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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NTS-PC1

Plasmid

#41574

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7 and Myc/Myc/His

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
PGK CFP

Plasmid

#200429

Purpose

Mammalian expression of CFP

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

CFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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CTS-PC1

Plasmid

#41576

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7 and myc/myc/his

Expression

Mammalian

Mutation

FLS (4184-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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hNedd4-2

Plasmid

#83433

Purpose

full length Nedd4L with His Xpress tag

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

Insert

human Nedd4L with C2 domain (NEDD4L Human)

Use

Tags

His Tag (6x) and Xpress Epitope Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Article

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Expression

Mutation

Promoter

T7

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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