-
Plasmid#172200PurposeAAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop C
Plasmid#171781PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-DogTag Loop C
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmBelle_1-298_B
Plasmid#145913PurposeInsect Expression of DmBelle_1-298DepositorInsertDmBelle_1-298 (bel Fly)
UseTagsExpressionInsectMutationdel 158-160 (GGG)PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42EF44A_C
Plasmid#146061PurposeInsect Expression of DmTral-R42EF44ADepositorInsertDmTral-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationR42E, F44A. Additionally, two silent mutations A1…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-EGFP-p16 shRNA
Plasmid#164919PurposeExpresses EGFP cDNA and p16 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_268-459_D
Plasmid#146119PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42E_B
Plasmid#145988PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2_209-791_A
Plasmid#145848PurposeInsect Expression of DmDCP2_209-791DepositorInsertDmDCP2_209-791 (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A149)
Plasmid#165998PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A149)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP-MIOX
Plasmid#166682PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-C-IDR
Plasmid#166441PurposeBacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion proteinDepositorInsertmEGFP, YAP-C-IDR(266-504) (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(E167)
Plasmid#166004PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(E167)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(D165)
Plasmid#166003PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(D165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only