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Plasmid#74905PurposeExpresses human Abi1 fused to GFPDepositorInsertAbi1 (ABL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
UseTagsHalo7 TagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4511NES
Plasmid#138375PurposeBooster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.DepositorInsertBooster-ERK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-Bsd-CMV-hUCK
Plasmid#195141PurposeUCK2 overexpression vector with blasticidin resistanceDepositorInsertUCK2 (UCK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTTN-1/1
Plasmid#91550PurposeProtein expression and purification of human SH3 domain construct CTTN-1/1DepositorInsertCTTN-1/1 (CTTN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2
Plasmid#136235PurposeExpresses human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
UseTagsStrep SumoExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-NES-YFP
Plasmid#127366PurposeBinary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-T2A-mRuby2
Plasmid#102989PurposeExpresses Cre and mRuby2DepositorInsertsCre (site-specific recombinase)
mRuby2
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 1
Plasmid#42540DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-NES-YFP
Plasmid#127369PurposeBinary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP/golgin-160/3DE
Plasmid#180631PurposeExpression of caspase-resistant golgin-160DepositorInsertgolgin-160/3DE (GOLGA3 Human)
UseTagsEGFPExpressionMammalianMutationD59E, D139E, D311EPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
Plasmid#89150PurposeAn AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.DepositorInsertHA-tagged hM4D(Gi)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-AMOT (p130)
Plasmid#172997Purposeconstitutive expression of V5-tagged AMOT (p130)DepositorInsertV5-AMOT (p130) (AMOT Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJA2
Plasmid#19519DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts2
Plasmid#115872PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts3
Plasmid#115873PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionTagsExpressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1328 - pAAV EF1a HA-hM3D(Gq)
Plasmid#89149PurposeAn AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.DepositorInsertHA-tagged hM3D(Gq)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only