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Plasmid#45205DepositorInsertHec1-targeted mCherry Plk1 T210D
UseTagsmCherryExpressionMammalianMutationPlk1 T210D mutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
PM-SP!TB
Plasmid#48650PurposeBacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicolDepositorInsertBacterial SP crRNA to prototspacer B
UseCRISPRTagsExpressionMutationPromoterJ23100 promoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoternEFAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAMQ79G:GlyR-IRES-GFP
Plasmid#32482DepositorInsertPSAMQ79G-GlyR
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJA258
Plasmid#21511DepositorInsert(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pDHJS4 AS+
Plasmid#78242PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS-
Plasmid#78245PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS+
Plasmid#78244PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AS-
Plasmid#78243PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS316 GAL cluster
Plasmid#61925PurposeExpression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS1
Plasmid#74887Purposetargeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5CDepositorInsertsAct5C upstream homology region
Act5C downstream homology
UseTagseGFPExpressionInsectMutationPromoterAvailable sinceApril 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPTg.T2FXK (#54)
Plasmid#35963DepositorInsertsTie2 promoter
Tie2 enhancer
UseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
DIG70 (T7->Rci; 11bc)
Plasmid#68835PurposeExpresses Rci gene (driven by the inducible T7 promoter) and 11 fragment barcode cassette in bacterial cellsDepositorInsertsBarcode cassette 11 fragments
Rci recombinase
UseTagsExpressionMutationPromoterT7Available sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-676 (attR4-attR3)
Plasmid#161888PurposeMultisite Gateway (attR4-attR3) vector for lentivirus productionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-686 (attR4-attR2)
Plasmid#161889PurposeMultisite Gateway (attR4-attR2) vector for lentivirus productionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
366 pTol2-CherryDest
Plasmid#73487Purposepromoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertmCherry-attR1/attR2
UseTagsExpressionMutationH30R (please see depositor comments below)PromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only