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Plasmid#175247PurposeBacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycinDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-spTorA-GFP(C)
Plasmid#169041PurposeGFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.DepositorInsertH6-spTorA-GFP
UseTags6xHis, TEV cleavage site, and spTorA (signal pept…ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR N52R (Shank3)
Plasmid#175255PurposeSPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with N52R mutation from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-13aa-Venus-pEGFP-C3
Plasmid#166753PurposeFor expression of mCerulean3 linked to Venus by 13 amino acid linkerDepositorInsertmCerulean3-13aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Noxa)-pEGFP-C1
Plasmid#166761PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of NoxaDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Noxa…PromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-39aa-Venus-pEGFP-C3
Plasmid#166755PurposeFor expression of mCerulean3 linked to Venus by 39 amino acid linkerDepositorInsertmCerulean3-39aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-hPMEL
Plasmid#174607Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitopeDepositorInsertexpresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-llo190-mc38minor
Plasmid#174604Purposeexpresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1DepositorInsertpalmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin(8A mut)
Plasmid#174707PurposeDoxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 bindingDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationS175A, T176A, T180A, S181A, S196A, T251A, T264A, …PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3 GFP pZa
Plasmid#170084PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR3Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-sfGFP-MiniWhite
Plasmid#165922PurposeBlasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-VHH[GFP]
Plasmid#163856PurposeZebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRXDepositorInsertVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2 GFP pZa
Plasmid#170081PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA2
Plasmid#171000PurposeHiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA6
Plasmid#171004PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only