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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MTOR gRNA (BRDN0001145868)

Plasmid

#76784

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N200Q/N219Q

Plasmid

#121454

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N200Q/N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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LATS2 gRNA (BRDN0001487153)

Plasmid

#77901

Purpose

3rd generation lentiviral gRNA plasmid targeting human LATS2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LATS2 (LATS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-ctrl

Plasmid

#169303

Purpose

Constitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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ROCK2 gRNA (BRDN0001146481)

Plasmid

#76350

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK2 (ROCK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK TT 3xFLAG ID2 neo

Plasmid

#98393

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ANLN

Plasmid

#31203

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ANLN (ANLN Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-LmoCas6

Plasmid

#126491

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

Plasmid

#157951

Purpose

Lentiviral expression of HBG site 1 sgRNA

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRalA-2

Plasmid

#15258

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-TRE-PtenWT-Blast

Plasmid

#135674

Purpose

Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7_hsa-miR-30c

Plasmid

#25797

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-30c

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pLJM60-S6K1(T389S) barcoded

Plasmid

#48800

Purpose

expresses mutant S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 T389S (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGTACC

Expression

Mammalian

Mutation

T389S

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pLenti4/V5-DEST-P85(KS459del)

Plasmid

#40222

Depositor

Lynda Chin

Insert

P85(KS459delN) (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

amino acids 459-460 replaced with asparagine

Promoter

CMV

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.shNkx2-1

Plasmid

#32400

Depositor

Monte Winslow, Tyler Jacks

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Nkx2-1 (Nkx2-1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
PRPS2 gRNA (BRDN0001147559)

Plasmid

#78005

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPS2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPS2 (PRPS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRPS2 gRNA (BRDN0001487102)

Plasmid

#78006

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPS2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPS2 (PRPS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_POU5F1_P2A_Hygro_Barcode

Plasmid

#120474

Purpose

Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU5F1 (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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